The National Center for Electron Cryomicroscopy at SOLARIS currently has two latest generation microscopes - Titan Krios and Glacios with the highest measurement parameters.

Titan Krios G3i

(Cryo-Transmission Electron Microscope)

1. Accelerating voltage 300 kV
2. Data allowing 3D reconstruction with ~ 2 Å resolution
3. Fully automatic, always ensuring optimal experimental conditions
4. Device compatibility allows contamination-free sample transfer between devices (Krios and Glacios)
5. Fixed power lenses reduce thermal drift and linear distortion is minimized to <1%
6. High measurement efficiency of up to 12 samples in a cluster, creation of overview atlases
7. It has a single-axis cryo-tomography holder
8. Equipped with, inter alia, three cameras: a 16-bit CMOS camera with a resolution greater than or equal to 14 MP and two cameras for direct detection of electrons with a resolution greater than or equal to 14 MP (Gatan K3, Thermofisher Scientific Falcon3)
9. Gatan GIF BioQuantum electron energy filter

Glacios

(Data collection & sample screening Cryo-Transmission Electron Microscope)

The purchase of the microscope and the IT infrastructure was financed by the European Union under Measure 4.2 of the Intelligent Development Operational Program 2014-2020.

1. Accelerating voltage 200 kV
2. The data allows reconstruction with a resolution of> 2.2 Å
3. Equipped with, inter alia, two cameras: a 16-bit CMOS camera with a resolution greater than or equal to 14 MP and a direct electron detection camera with a resolution greater than or equal to 14 MP (Thermofisher Scientific Falcon4). 

 

 

 

Access rules for microscopes

Glacios

The Center is providing paid access to the Glacios microscope for commercial and non-commercial users. However preferential rates are applied for the researchers with non-commercial purposes. In case the number of research projects submitted by the scientific community exceeds the capacity of the infrastructure, it is planned to incorporate the procedure of substantive assessment of submitted ideas.

Access to the infrastructure in the economic part is based on commercial principles, and the price of services is determined on the basis of competitive analysis, taking into account such factors as the quality of infrastructure and the experience and qualifications of staff. Access is provided on a first-come, first-served basis, and in the case of high interest, with pre-selection of applications based on substantive criteria.

For more information about the access and prices please contact Dr. Piotr Ciochoń (Industry Liaison Officer) at industry.solaris@uj.edu.pl

For information on the feasibility of the experiment please contact Dr. Michał Rawski (Microscope Operator) at michal.rawski@uj.edu.pl

 

Titan Krios

For access to the Titan Krios microscope, it is necessary to apply for research time through the DUO User Support System (https://duo.synchrotron.pl/) or through the CERIC-ERIC consortium website (https://www.ceric-eric.eu/users/login-to-vuo/). 

Applications can be submitted twice a year in Spring and Autumn calls. More information about the upcoming call HERE.