This imaging method is based on the elastic scattering of an electron beam by thin films of studied materials, or by separate molecules, and is capable of reaching atomic resolution.
It allows scientists to solve atomic structures of organic molecules and macromolecules without crystallization. The samples are usually frozen in liquid ethane and their 3D structure is reconstructed based on the semi-automated analysis of hundreds of microscopic images, showing 2D projections of randomly oriented molecules.
CryoEM can be used in the following industry sectors:
• Biotechnology, structural biology (atomic structure of proteins and other biomolecules)
• Pharmaceutical (drug discovery and development, atomic structure of small organic molecules, active substances, complexes, drug delivery systems)
• Cosmetics (migration of cosmetics through skin, nails, hair structure, uptake of active substances by cells)
• New materials and nanotechnology (atomic structure of materials, nanoparticles, structure of low-dimensional materials, such as graphene, crystallization mechanisms, optimization of production processes)
• Energy (structure of porous materials, used as electrodes, or for hydrocarbon/hydrogen storage)
CryoEM measurements are performed on the Thermo Scientific™ Glacios™ microscope utilizing accelerating voltage of 80-200kV, equipped with the latest generation Falcon 4EC detector, which allows for achieving the spatial resolution down to 2.2Å.
We offer both basic imagings, detailed sample characterization service and the full reconstruction of the structure of biomolecules.
