The ARYA (mAcromolecular x-RaY crystAllography) is an advanced research infrastructure designed to utilize high-energy synchrotron radiation for structural analysis of proteins, nucleic acids, and small molecules. Its primary goal is to support research in structural biology, chemistry, and pharmaceutical sciences.

The MX end station has been designed for maximum flexibility and precision. It will enable:

• wavelength selection for MAD (Multiwavelength Anomalous Diffraction) experiments,
• high-flux operation for routine diffraction data collection from protein crystals.

Automation & Experimental Diversity

The ARYA beamline will be fully automated, allowing a wide range of experiments, including:

• standard diffraction data collection at room temperature and cryogenic conditions (80–100 K),
• helical data collection,
• raster scanning of crystals to locate optimal diffraction regions,
• high-pressure experiments using Diamond Anvil Cells (DACs).

Advanced Instrumentation

The end station will be equipped with:

• a fast and precise microdiffractometer,
• an autosampler accommodating approximately 400 samples,
• a hybrid photon-counting detector,
• a fluorescence detector for identifying metal-containing samples.

Users will be able to conduct experiments entirely remotely, without the need for physical presence at the facility.

Software & Data Analysis

Comprehensive software will be installed for data collection, processing, and analysis, enabling:

• real-time monitoring of experimental results,
• recording of data collection parameters and metadata,
• efficient management of the research workflow.

Laboratory infrastructure

The ARYA beamline will also include dedicated laboratory facilities to support comprehensive sample preparation, including:

• protein expression and purification,
• crystallization and storage of crystallization plates,
• automated imaging with crystal/salt discrimination.

Future developments will include fragment screening experiments, both crystallographic and interaction-based.

Beamline parameters

Parameters	Value
Source	3-pole wiggler
Available energy range	4-22 keV
Energy resolution ΔE/E	DCM 1 x10-4; DMM 1 x10-2
Beam size at sample (H x V)	260 x 80 µm
Photon flux at sample	~1012 ph/s
End station	MX

 

Construction of the ARYA beamline and the start of user experiments are planned for late 2027.